Journal:

Article Title: Effects of Antenatal and Postnatal Environments on CD4 T-Cell Responses to Mycobacterium bovis BCG in Healthy Infants in The Gambia ▿

doi: 10.1128/CVI.00037-08

Figure Lengend Snippet: Gating strategy used to define responding cells. Following gating on lymphocytes expressing high levels of CD4, cross-hairs were set on NHDF-stimulated cells (A) and applied to PPD-stimulated cells (B). The representative sample is from a 12-month-old infant in whom the relatively low percentage of IL-2-producing cells and tendency for coexpression of IFN-γ and CD154 are evident. Percentages refer to events in the labeled quadrant.

Article Snippet: The PBMCs were resuspended at 2 × 10 6 cells ml −1 in R10F (90% [vol/vol] RPMI 1640 containing 100 U ml −1 penicillin plus 100 μg ml −1 streptomycin, 10% [vol/vol] fetal bovine serum) and treated with either 10 μg ml −1 of the RT49 preparation of tuberculin purified protein derivative (PPD) (Statens Serum Institut) or 10 μg ml −1 normal human dermal fibroblast lysate (NHDF) (Virusys) as a negative control, which was required by the CMV study.

Techniques: Expressing, Labeling

Journal: International Journal of Molecular Sciences

Article Title: A New Bone Substitute Developed from 3D-Prints of Polylactide (PLA) Loaded with Collagen I: An In Vitro Study

doi: 10.3390/ijms18122569

Figure Lengend Snippet: Microscopic analysis of different cell types seeded on PLA discs. ( A ) hOB (human primary osteoblasts; ( B ) normal human dermal fibroblasts: NHDF; ( C ) human umbilical vein endothelial cells: HUVEC; and ( D ) osteosarcoma cells: SaOS-2. Scale bars represent 400 µm.

Article Snippet: Normal human dermal fibroblasts (NHDF, Promega, Karlsruhe, Germany), human primary osteoblasts (hOB [ , ]), osteoblast-like cells (SaOS, ATCC, Manassas, VA, USA) and endothelial cells (HUVEC, Promega, Karlsruhe, Germany) were seeded onto discs, into or onto cages, respectively, sitting in a 24-well ultra-low attachment plate.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: A New Bone Substitute Developed from 3D-Prints of Polylactide (PLA) Loaded with Collagen I: An In Vitro Study

doi: 10.3390/ijms18122569

Figure Lengend Snippet: Proliferation assays on PLA discs with different cell types. ( A ) without collagen; ( B ) PLA discs coated with rat tail collagen; ( C ) PLA discs coated with bovine collagen. Blue: HUVEC, red: NHDF, green: hOB and lilac: SaOS-2.

Article Snippet: Normal human dermal fibroblasts (NHDF, Promega, Karlsruhe, Germany), human primary osteoblasts (hOB [ , ]), osteoblast-like cells (SaOS, ATCC, Manassas, VA, USA) and endothelial cells (HUVEC, Promega, Karlsruhe, Germany) were seeded onto discs, into or onto cages, respectively, sitting in a 24-well ultra-low attachment plate.

Techniques: